Andrew Deegan, Rory Scholes and captain JP Cooney are among the players drafted into the side.
Once again, the strong selection includes a mix of senior and Connacht Academy players as Ireland international Niyi Adeolokun and Fiji international Naulia Dawai both maintain their places.
Out-half Deegan partners Conor McKeon at half-back with Scholes starting on the left wing as Stacey Ili maintains his place at outside centre.
The Connacht Academy, sponsored by NUIG, is well represented with Matthew Byrne at full back, Kieran Joyce at inside centre, Peter Claffey in the second row, and both Paul Boyle and Harley Fox in the back row.
An all-senior front row sees Denis Coulson and Cooney flank Pat O’Toole with lock Gavin Thornbury partnering Claffey in the engine room.
Scrum-half James Mitchell is set to make his return from injury off the bench with three Academy players among the replacements – out-half Luke Carty, back three player Adam Leavy and prop Conor Kenny.
Ireland U18 internationals Cian Huxford and Callum Torpey (Exile) complete the bench along with Niall Murray, last year’s Connacht U18 Player of the Year.
It is set to be a very tough task against an Ealing Trailfinders side that are flying high in the Championship with five wins from six.
They made a superb start to the B&I Cup campaign thanks to a 67-12 win over Richmond last week and former Connacht out-half Shane O'Leary is among their ranks.
Admission to this game for Season Ticket holders and under-12s is free of charge.
General admission is €5.
Connacht Eagles: Matthew Byrne; Niyi Adeolokun, Stacey Ili, Kieran Joyce, Rory Scholes; Andrew Deegan, Conor McKeon; Denis Coulson, Pat O’Toole, JP Cooney (Capt); Peter Claffey, Gavin Thornbury; Paul Boyle, Naulia Dawai, Harley Fox.
Replacements: Callum Torpey, Conor Kenny, Niall Murray, Cian Huxford, James Mitchell, Luke Carty, Adam Leavy.
Connacht Eagles B&I Cup Fixtures & Results
Saturday, October 14
Rotherham 28-23 Connacht Eagles, Clifton Lane;
Sunday, October 22
Connacht Eagles v Ealing Trailfinders, The Sportsground, 2pm;
Saturday, December 9
Richmond v Connacht Eagles, Athletic Ground, 3pm;
Sunday, December 17
Connacht Eagles v Richmond, The Sportsground, 2pm;
Saturday, January 13
Ealing Trailfinders v Connacht Eagles, Trailfinders Sports Ground, 3pm;
Weekend of Saturday, January 20
Connacht Eagles v Rotherham, The Sportsground, TBC;